Nā huahana

Huahana Huahana:

Hana ʻia ʻo Innovita® 2019-nCoV IgM/IgG Test no ka ʻike semi-quantitative o ka neutralizing antibodies i ka novel coronavirus (2019-nCoV) i loko o ke kanaka serum, plasma a i ʻole nā ​​​​mea kikoʻī koko holoʻokoʻa.
Loaʻa i ka 2019-nCoV ʻehā mau pūmua kumu nui: S protein, E protein, M protein a me N protein.Hiki i ka ʻāpana RBD o ka protein S ke hoʻopaʻa i ka mea hoʻopaʻa i ka ʻili o ke kanaka ACE2.ʻO ka neutralizing antibody e pili ana i ka hiki ke hoʻopaʻa me ka pathogen, a laila pale i ka pathogen e hoʻouka i ke kino e hoʻokau i ka maʻi.Hiki ke hoʻohana ʻia ka ʻike ʻana o ka neutralizing antibody e loiloi i ka wānana o ka maʻi viral.

Kumu:

ʻO ka pahu he colloid gula immunochromatography hoʻokūkū hoʻokūkū e ʻike ai i ka neutralizing antibodies i 2019-nCoV i loko o ke kanaka serum, plasma a i ʻole nā ​​​​koko koko holoʻokoʻa.Ma hope o ka hoʻopili ʻia ʻana o ka mea hoʻohālike i ka pūnāwai, inā loaʻa nā antibodies neutralizing i loko o ka specimen, e pane nā antibodies neutralizing me ke gula colloidal i kapa ʻia ʻo RBD antigen e hana i ka paʻakikī immune, a e pani ʻia ka wahi neutralizing o ka antigen RBD i kapa ʻia.A laila, neʻe ka paʻakikī immune a me ka antigen RBD i kapa ʻia me ka hoʻopaʻa ʻole ʻana i ka antibody neutralizing ma ka membrane nitrocellulose.Ke hōʻea lākou i ka ʻāpana hoʻāʻo (laina T), ʻo ka RBD antigen i hoʻopaʻa ʻole ʻia me ka hoʻopaʻa ʻole ʻana i nā antibodies e hoʻoneʻe ʻia me ka antigen ACE2 i uhi ʻia ma ka membrane nitrocellulose a hana i kahi laina ʻulaʻula-ʻulaʻula.Ke ʻoi aku ka kiʻekiʻe o ka neʻe ʻana o nā antibodies neutralizing ma mua o ka palena ʻike haʻahaʻa, ʻoi aku ka māmā o ka laina ʻulaʻula ma mua o ka laina mana (laina C) a i ʻole ʻaʻohe laina ʻulaʻula-ʻulaʻula i hana ʻia, maikaʻi ka hopena.Ke emi iho ka neʻe ʻana o nā antibodies neutralizing ma mua o ka palena haʻahaʻa haʻahaʻa a i ʻole ʻaʻohe ʻano antibody neutralizing i ka specimen, ʻoi aku ka ʻeleʻele o ka laina poni-ʻulaʻula ma mua o ka laina hoʻomalu, ʻaʻohe hopena.
ʻAʻole paha inā he 2019-nCoV neutralizing antibodies i ka specimen, i ka wā e neʻe ai ka moa colloidal gula-labeled IgY antibody i ka laina control (laina C), e hopu ʻia ia e ka anti-chicken antibody IgY antibody i hoʻopaʻa mua ʻia ma ka laina control (C). laina), hana ʻia kahi laina ʻulaʻula.Hoʻohana ʻia ka laina mana (laina C) ma ke ʻano he kaʻina hana.Pono e ʻike mau nā laina mana i nā puka makani hopena inā hana pono ke kaʻina hana hoʻāʻo a ke hana nei nā reagents e like me ka mea i manaʻo ʻia.

Huina:

Kaʻina hana hoʻāʻo:

1. Wehe i ka ʻeke alumini alumini a lawe i waho i ka pahu hoʻāʻo.
2. E noi i ka 40μL o ka serum / plasma specimen a i ʻole 60μL ke koko holoʻokoʻa i ka pūnāwai.
3. E noi i ka 40μL (2 kulu) specimen diluent i ka pūnāwai specimen.
4. E kau i ka lumi wela (15 ℃ ~ 30 ℃) no nā minuke 15-20, a heluhelu i ka hopena.

Nā hualoaʻa wehewehe:

1. Maikaʻi: Inā ʻoi aku ka māmā o ke kala o ka laina T ma mua o ko ka laina C a i ʻole ka laina T, hōʻike ia i ka maikaʻi no ka pale ʻana i nā antibodies.
2. ʻAʻohe: Ke ʻeleʻele ke kala o ka laina T ma mua a i ʻole like me ko ka laina C, hōʻike ia i ka maikaʻi ʻole no ka pale ʻana i nā antibodies.
3. He kūpono ʻole: Ke ʻike ʻole ʻia ka laina C, inā ʻike ʻia ka laina T a ʻaʻole paha, ʻaʻole kūpono ka hoʻāʻo.E hana hou i ka ho'āʻo me kahi hoʻāʻo hou.