2019-nCoV Neutralizing Antibody Test （QDIC）
Huahana Huahana:
Manaʻo ʻia ʻo Innovita® 2019-nCoV IgM/IgG Test no ka ʻike nui ʻana o ka neutralizing antibody i ka novel coronavirus (2019-nCoV) i loko o ke kanaka serum, plasma a i ʻole ke koko holoʻokoʻa (koko manamana lima a i ʻole venous koko holoʻokoʻa).
Loaʻa i ka 2019-nCoV ʻehā mau pūmua kumu nui: S protein, E protein, M protein a me N protein.Hiki i ka ʻāpana RBD o ka protein S ke hoʻopaʻa i ka mea hoʻopaʻa i ka ʻili o ke kanaka ACE2.Ua hōʻike ʻia nā haʻawina he maikaʻi nā ʻano o nā poʻe i hoʻōla mai ka maʻi coronavirus novel no ka neutralizing antibody.Hiki ke hoʻohana ʻia ka ʻike ʻana i ka antibody neutralizing e loiloi i ka wānana o ka maʻi viral a me ka loiloi hopena ma hope o ka hoʻopaʻa ʻana.
Kumu:
ʻO ka pahu he quantum dot immunofluorescence chromatography assay e ʻike ai i ka 2019-nCoV RBD kikoʻī IgG neutralizing antibodies i loko o ka serum kanaka, plasma a i ʻole ke koko holoʻokoʻa (koko manamana lima a me ke koko holoʻokoʻa venous).Ma hope o ka hoʻopili ʻana o ka mea hoʻohālike i ka pūnāwai, inā ʻoi aku ka kiʻekiʻe o ka neʻe ʻana o nā antibodies neutralizing ma mua o ka palena haʻahaʻa haʻahaʻa loa, e pane ka RBD specific IgG antibodies me kahi ʻāpana a i ʻole nā antigen RBD āpau i hoʻopaʻa ʻia me nā microspheres quantum dot e hana i ka pūhui immune.A laila e neʻe ka pūhui immune ma ka membrane nitrocellulose.Ke hōʻea lākou i ka ʻāpana hoʻāʻo (laina T), e pane ka hui me ka IgG anti-human kanaka (γ chain) i uhi ʻia ma ka membrane nitrocellulose a hana i kahi laina fluorescent.E heluhelu i ka waiwai hōʻailona fluorescence me ka mea hōʻike immunoassay fluorescence.Hoʻohālikelike ka waiwai hōʻailona i ka ʻike o ka neutralizing antibodies i ka specimen.
Inā loaʻa i ka specimen nā antibodies neutralizing kikoʻī RBD a ʻaʻole paha, pono e ʻike mau ʻia ka laina hoʻomalu ma ka puka makani hopena inā hana pono ke kaʻina hana hoʻāʻo a ke hana nei ka reagent e like me ka mea i manaʻo ʻia.Ke neʻe ka moa IgY antibody i kapa ʻia me nā microspheres quantum dot i ka laina hoʻomalu (laina C), e hopu ʻia ia e ka anti-moa antibody IgY i hoʻopaʻa mua ʻia ma ka laina C, a ua hoʻokumu ʻia kahi laina fluorescent.Hoʻohana ʻia ka laina mana (laina C) ma ke ʻano he kaʻina hana.
Huina:
| Huina | Ka nui | Hōʻike |
| IFU | 1 | / |
| Pīkeke hoʻāʻo | 20 | ʻO kēlā me kēia ʻeke pepa i hoʻopaʻa ʻia me hoʻokahi mea hoʻāʻo a me hoʻokahi desiccant |
| Mea hoʻoheheʻe ʻia | 3mL*1 hue | 20mM PBS, Sodium Casein, ProClin 300 |
| Micropipette | 20 | Micropipette me 20μL laina hoailona |
| Lancet | 20 | / |
| Pahu waiʻona | 20 | / |
Kaʻina hana hoʻāʻo:
● ʻOhi Koko manamana lima
● Heluhelu i ka hopena me ka mea hōʻike fluorescence
