Nā huahana

Huahana Huahana:

Hana ʻia ʻo Innovita® 2019-nCoV IgM/IgG Test no ka maʻi a me ka nānā ʻana i nā maʻi maʻi i hoʻokumu ʻia e ka novel coronavirus (2019-nCoV).ʻIke ʻia ʻo ORF1ab a me N gene o 2019-nCoV mai ka ʻāʻī ʻāʻī a me nā ʻāpana wai holoi alveolar i hōʻiliʻili ʻia mai nā maʻi i manaʻo ʻia o ka maʻi pneumonia, nā mea maʻi me nā hihia kānalua, nā poʻe ʻē aʻe e pono e ʻike.
ʻO nā hopena hoʻāʻo o kēia kit no ka hōʻike lapaʻau wale nō.Manaʻo ʻia e hana i kahi loiloi piha o ke ʻano ma muli o nā hōʻike hōʻike hōʻike o ka mea maʻi a me nā hoʻokolohua ʻē aʻe.

Kumu:

Hoʻohana kēia pahu i hoʻokahi ʻanuʻu hoʻohuli hoʻohuli polymerase chain reaction (RT-PCR) ʻenehana ʻike maka no ka hoʻopaʻa ʻana i ka novel coronavirus (2019-nCoV) ORF1ab gene, N gene, a me ke kaʻina moʻo kuhikuhi kūloko kanaka.Ua hoʻolālā ʻia nā kumu mua a me ka taqman probes ma nā wahi i mālama ʻia.

Huina:

'Ōlelo Aʻo: 1. ʻAʻole pono e hui ʻia nā ʻāpana like ʻole o nā reagents.
2. ʻAʻole pono e unuhi ʻia nā mana maikaʻi a me nā mana maikaʻi

Kaʻina hana hoʻāʻo:

1. Nucleic acid extraction:
Loaʻa nā pahu hoʻoheheʻe RNA kālepa, ʻae ʻia ka ʻili ʻana i ka bead magnetic a me ka ʻoki kolamu spin no kēia kit.
2. E hoʻomākaukau i ka Reaction Mix:

● Wehe i ka hopena 2019-nCoV Mix A/B a mālama i ka lumi wela a hiki i ka hoʻoheheʻe ʻana;
● E lawe i nā ʻāpana like (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) a hui pū, a laila aliquot kēlā me kēia PCR pane me 20μL/ tube;
● Hoʻohui i ka 5μL o RNA template a i ʻole ka mana maikaʻi ʻole a i ʻole ka mana maikaʻi, a laila e uhi i ka pāpale paipu;
● E kau i ka paipu hopena i loko o ka mea hana PCR fluorescence, a hoʻonoho i ka mana maikaʻi ʻole / maikaʻi a me nā ʻāpana hoʻohālike no ka pane ʻana o RT-PCR e like me nā ʻōlelo aʻoaʻo hana.
● E hoʻopaʻa i ke kauoha hoʻonoho laʻana

3. RT-PCR protocols:

Koho ʻia: